WebSep 16, 2013 · The predominant factor differentiating non-specific binding of fluorescent conjugates to the same substrate was shown to be the dye used. In this study we have compared a much larger range of dyes that can be excited with the commonly used 491 nm, 561 nm and 638 nm laser lines on a single substrate and conjugated to a single ligand of … WebA patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR. Highly concentrated for customers with specialized applications requiring a concentrated dye solution. Quick Add Detailed View Product MB Acid From: $275 Sizes: 5 mg Catalog #: 40076
The Non-Specific Binding of Fluorescent-Labeled MiRNAs on Cell …
WebFeb 15, 2004 · Binding of fluorescent dye to genomic RNA inside intact human rhinovirus after viral capsid penetration investigated by capillary electrophoresis Authors Leopold … WebAdd products for $400 to your cart and get free express shipping . Fluo-4 AM is a cell-permeable Ca 2+ -indicator that is metabolized by intracellular esterase, leading to a bright green fluorescent signal upon Ca 2+ -binding (excitation/emission λ at 494/506 nm). Fluo-4 AM is used for quantifying intracellular Ca 2+ concentrations. imagination dance woodstock
Dye-doped silica nanoparticles: synthesis, surface chemistry and ...
WebThe BAPTA-based indicators, including the Calcium Green dyes, fluo-3 and fluo-4, exhibit the highest emission intensities upon binding La 3+, Hg 2+ and Cd 2+. The response of the Calcium Green-1 and Magnesium Green indicators shows … WebMar 13, 2024 · To overcome these limitations, we developed three novel extracellular vesicular-membrane-binding fluorescent probes—Mem dye-Green, Mem dye-Red, and Mem dye-Deep Red—for monitoring EV uptake into cells. The dyes contain a cyanine group as a fluorescent scaffold and amphiphilic moieties on the cyanine. WebMar 16, 2024 · Fluorescent dyes have been used in quantitative PCR (qPCR) for many years ().The most commonly used of these dye is the cyanine intercalating dye SYBR Green I (SG) ().SG is known for its DNA binding sensitivity and is reported to have an ∼1000-fold increase in fluorescence when bound to DNA (3, 4).Other fluorescent dyes … list of english counties 2020