Web5. Add CytoTracker™ to a final concentration of 1X (for example, add 2 µL of 500X CytoTracker™ solution to 1.0 mL of cancer cell suspension). Incubate for 60 min at 37ºC in a cell culture incubator. Spin down cells at 1000 rpm for 2 minutes, aspirate the medium and wash cell pellet with serum free media. Repeat the wash twice. WebGiven the severity of the COVID-19 outbreak, Chroma's top priority remains the health and well-being of our employees, customers and greater communities.
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WebMar 28, 2024 · Biolayer interferometry analysis of TAT-CaM binding to CBS-E2. (A) Association-then-dissociation experiment in which ligand TAT-CaM was exposed to varying concentrations of CBS-E2 prior to movement to buffer only at 90s (red, 1000 nM; orange, 500 nM; yellow, 250 nM, green, 125 nM, blue 63 nM). Data points are individual … WebNov 7, 2005 · The mitochondrion-selective probe MitoTracker Orange was obtained from Invitrogen. Apoptosis assays. To examine cell death resulting from cell adhesion, cells were plated in medium supplemented with 0.5% FBS on 35-mm Petri dishes precoated overnight with various proteins. After 24 h of incubation, cells were fixed with a 10% formaldehyde ... chevy cch
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WebDec 4, 2024 · The cells were pelleted by centrifugation at 500 x g for 5 min at 4 °C, and the cells were resuspended in 2 mL Wash Buffer containing 10 μM CytoTracker Orange (also known as CMTMR; ThermoFisher; stock solution 10 mM in DMSO) and incubated at 37 °C for 30 min. The CMTMR-labelled cells were washed 2 times in 25 mL Wash Media and … WebJan 3, 2012 · Adherent PECs were demarcated into regions of interest based on positive CytoTracker Orange staining. The mean pixel intensity (PI) of Alexa Fluor 488-labeled Ag fluorescence within the cell boundary was calculated. A background region of interest was distinguished for each frame, and the mean background signal was subtracted from the ... WebNov 10, 2024 · ( i) presents a three-line structure composed of alternating printed stripes of HaCaT and A431 cells, labelled with cytotracker-green and cytotracker-red respectively. This structure was... chevy cavalier z24 convertible for sale