WebJan 1, 2006 · Fut8 Activity Assay The specific activity of Fut8 was determined using a synthetic substrate, 4‐(2‐pyridylamio)‐butyl‐amine (PABA)–labeled oligosaccharide as a … WebAug 29, 2024 · Serum and urine FUT8 activity were measured using HPLC, as previously described. 12, 13 A serum or urine sample was added to a mixture of 2 …
An Assay for α 1,6-Fucosyltransferase (FUT8) Activity
WebFeb 20, 2024 · FUT8 is the only enzyme responsible for core-fucosylation on mammalian N -glycoproteins. Despite many years of research on the … WebJan 27, 2024 · p cFUT8 attenuated the inhibitory effects of miR-548c on FUT8 expression, examined by RIP. q Dual-Luciferase Reporter Assay System showed that miR-548c … pantone 19-4215 tcx
(PDF) Functional knockout of FUT8 in Chinese hamster
Fut8 activity was determined by a synthetic substrate, 4-(2-pyridylamin)-butyl-amine (PABA)-labeled oligosaccharide as a substrate. Sample from lysed cell or tissue was suspended in a 200 μl 1× RIPA buffer containing 10 mM Tris–HCl, pH 7.4, 150 mM NaCl, and 1 % Triton X-100. The assay of Fut8 activity was … See more The cells were seeded onto a 60-mm culture dish at 5 × 105 and incubated for 24–48 h. After washing twice with cold PBS (−) containing … See more The cell was cultured on 24-well plates at 1.5 × 105 per well. After washing twice with 500 μl cold PBS (−) containing 0.1% BSA, and then incubated in 200 μl of PBS (−) containing 125I-TGF-β1 in a concentration range … See more WebJul 1, 2024 · The FUT8 activity assay showed that the deletion of residues 1–67 and residues 1–108 had no effect on the activity. However, further deletion resulted in the … WebAn Assay for α 1,6-Fucosyltransferase (FUT8) Activity Based on the HPLC Separation of a Reaction Product with Fluorescence Detection Authors: Hideyuki Ihara 1 , Hiroki … pantone 19-4326 tcx