WebMar 25, 2014 · In turn, basal body localization of Dido3 and HDAC6 depends on the actin network, previously shown to limit cilium size independent of the cell cycle. ... resulting in a truncated protein termed ... WebSep 27, 2002 · In a directed yeast two-hybrid assay, truncation mutants (Fig. 3A) comprising amino acids 1–830, 1–953, and 1–1111 all failed to bind polyubiquitin (Fig. …
Inhibition of HDAC6 activity protects dopaminergic …
WebDec 23, 2024 · However, HDAC6 in the healthy CNS functions via distinct mechanisms, due largely to its cytoplasmic localization. Indeed, in healthy neurons, cytoplasmic HDAC6 regulates the acetylation of a variety of non-histone proteins that are linked to separate functions, i.e., intracellular transport, neurotransmitter release, and aggregate formation. WebMay 24, 2013 · Truncation of the SE14 domain (Ala-883 to Pro-962) of HDAC6 led to significantly impaired binding of mutant SOD1 (Fig. 2B, lane 8). Likewise, the in-frame deletion of the SE14 domain in the context of full-length HDAC6 also weakened the coprecipitation with mutant SOD1 ( Fig. 2 B , lane 9 ). main lord of the rings characters
The disordered N-terminus of HDAC6 is a microtubule-binding …
WebTo define the domain(s) required for interaction between HDAC6 and G3BP, truncated proteins were expressed by transfection in 293T cells, and interaction was tested by coimmunoprecipitation assays. As shown in Figure 2,A and B , the full-length HDAC6 protein as well as deletion mutants ( Seigneurin-Berny et al. 2001 ) containing at least … WebDec 2, 2024 · HDAC6 is a member of HDAC family, which plays an important role in multiple diseases, with its role in gastrointestinal cancer being prominent. Generally, it could be considered as an oncogenic factor in esophageal, colorectal, and pancreatic cancers; however, its role in GC, liver cancer, and CCA remains controversial. WebFeb 2, 2012 · To identify the GRK2-binding region in HDAC6, a battery of HA-tagged HDAC6 truncated constructs was used (Figure 2D). ... HDAC6 and GRK2 colocalize in the leading edge of migrating cells. HeLa cells stably expressing GRK2-wt were plated in FN (10 μg/ml)-coated dishes and scratched to promote wound healing as indicated in Materials … main lounge at arboretum