Mcherry-egfp-lc3

Author: ukaj

August undefined, 2024

WebpCMV-mCherry-p62是碧云天研发的在哺乳动物细胞中表达红色mCherry标签的人源p62融合蛋白的质粒。该质粒含有CMV启动子，为卡那霉素抗性，转染后能够在靶细胞中高效表 … WebWe detail here a protocol using tandem-tagged mCherry-EGFP-LC3 (C-G-LC3) to quantify autophagic flux in single cells by ratiometric flow cytometry and to isolate subpopulations …

pCMV-GFP-LC3B(pCMV-GFP-LC3B质粒)(D2815-1μg)

WebDownload scientific diagram Use of mCherry-EGFP-LC3 as a fluorescent marker for autophagy execution in skeletal muscle (A) 10 days after electroporation of the mCherry-EGFP-LC3 cDNA plasmid into ... WebPlasmid FUW mCherry-GFP-LC3 from Dr. Anne Brunet's lab contains the insert mCherry-GFP-LC3 and is published in Science. 2024 Mar 16;359(6381):1277-1283. doi: … industry life cycle examples

Hypoxia promotes noncanonical autophagy in nucleus pulposus …

Web19 nov. 2024 · The effect of genetic and pharmacologic inhibition of ERK on GBM growth in vitro and in vivo was researched. An image-based tracking analysis of LC3 using mCherry-eGFP-LC3 plasmid, and transmission electron microscopy were utilized to monitor autophagic flux. Immunoblot analysis was used to measure the related proteins. Web12 apr. 2024 · SH-SY5Y and HEK293T cells engineered with mCherry-GFP-LC3 were subjected to treatment with the antidepressant amitriptyline, the autophagy activator rapamycin, and the autophagy inhibitors MG132 and ammonium chloride followed by the analysis of macroautophagy and CMA markers to monitor the autophagy process at … WebMcherry Egfp Lc3 Dual Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … login agent service account

R26-CAG-LSL-mCherry-EGFP-LC3 - Model Org

Autophagy deficiency exacerbates iron overload induced reactive …

WebWe used the mCherry-EGFP-LC3 construct and FACS analysis to compare the autophagic flux induction in these two cell lines upon Lapatinib treatment. We observed a significantly higher basal autophagic flux in OE19 LR compared to OE19 P cells. Moreover, Lapatinib treatment resulted in a significant induction of flux in both lines (Figure 2b). Webmcherry-GFP-Lc3 reporter cells enable flow cytometric quantification and sorting of cells based on autophagic flux. (A) Quantification of autophagic flux by flow cytometry using … login agentWebThe number of GFP puncta is counted before and after stimulation. (2) tfLC3 mice express RFP-EGFP-LC3 or mCherry-EGFP-LC3 for measuring autophagy ﬂux. The num-bers of … login agency zoom

"WebPancreatitis is a common, sometimes fatal, disease of exocrine pancreas, initiated by damaged acinar cells. Recent studies implicate disordered macroautophagy/autophagy … " - Mcherry-egfp-lc3

Mcherry-egfp-lc3

Transgenic expression of GFP-LC3 perturbs autophagy in

Web7 nov. 2015 · Both clones expressed 75kDa mCherry-EGFP-LC3 fusion protein, while clone relativelylow expression level (Fig. 1C). faintband 25kDa detected westernblots may represent free EGFP protein. freeEGFP synthesizeddur- ing translation because EGFPstart stopcodons morelikely producedfrom lysosomal degradation tfLC3B(Hosokawa et al., ...

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WebImage: Illustrated plasmid map in PNG format GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene … WebPancreatitis is a common, sometimes fatal, disease of exocrine pancreas, initiated by damaged acinar cells. Recent studies implicate disordered macroautophagy/autophagy in pancreatitis pathogenesis. ATG8/LC3 protein is critical for autophagosome formation and a widely used marker of autophagic vacuo …

Web8 jul. 2024 · 1. Generation of autophagy reporter cell line of immortalized human chondrocytes. NOTE: The generation of an autophagy reporter cell line by stable transfection of pBABE-mCherry-GFP-LC3 by establishing a flow cytometry quantitative readout was described previously 11.Two cell lines were used in the retroviral … WebChronic alcohol consumption retards lipophagy, which contributes to the pathogenesis of liver steatosis. Lipophagy-related Rab7 has been presumed as a crucial regulator in the progression of alcohol liver disease despite elusive mechanisms. More importantly, whether or not hepatoprotective quercetin targets Rab7-associated lipophagy disorder is …

WebIntroduction. An often-overlooked feature of the metabolic syndrome is the common occurrence of iron overload, particularly upon aging [].Indeed, the term dysmetabolic iron overload syndrome (DIOS) has been coined to describe the association of increased iron levels, characterized by hyperferritinemia with normal or moderately increased transferrin … Web24 jun. 2024 · Cells stably expressing mCherry-GFP-LC3 (to measure autophagy), mCherry-GFP-Fis1 (to measure mitophagy based on degradation of the outer mitochondrial membrane), mito-Keima (to measure mitophagy based on degradation of the inner mitochondrial membrane), or mCherry-GFP-SNX9 (to measure flux of SNX9 through …

WebC3003-100μl. Lenti-EGFP-LC3B (10^9TU/ml) 100μl. 2992.00元. Lenti-EGFP-LC3B，即Lentivirus expressing EGFP-LC3B fusion protein，是一种可以表达EGFP-LC3B融合蛋白的慢病毒，可以用于感染细胞或组织后进行细胞自噬 (autophagy)检测。. 本产品带有嘌呤霉素 (puromycin)抗性，感染细胞后可以筛选 ...

Web19 feb. 2024 · Tandem mCherry-GFP-LC3 and ratiometric flow cytometry A more quantitative assay to measure autophagy couples the tandem mCherry-GFP-LC3 construct with ratiometric flow cytometry 3 . Images … log in agent services accountWebDownload scientific diagram The GFP and mRFP signals of tfLC3 show different localization patterns. (A) Diagram of tfLC3 structure. (B) HeLa cells were cotransfected with plasmids expressing ... industry life cycle frameworkWeb,南模生物是国内模式生物领域领先的生物科技公司，专业从事遗传修饰动物模型(转基因小鼠、斑马鱼、线虫)的研发、饲养 ... industry life cycle journal pdfWeb17 sep. 2024 · The mCherry-GFP-LC3 model allowed us to demonstrate that H. pylori infection reduced the autophagic flux, meaning that a part of autophagy remains incomplete, without content degradation, while another part of autophagy remains complete with content degradation. Autophagy process sustains the EMT-like phenotype induced by H. pylori log in agricoachingWeb10 apr. 2024 · Main. Macro-autophagy (hereafter termed ‘autophagy’) encapsulates large intracellular cargos in a double-membrane structure named the autophagosome 1, 2. Upon fusion of the autophagosome with ... industry life cycle of amazonWebGeneMedi's lentivirus CMV expression vector is inserted with a promoter for cytomegalovirus (CMV) to drive gene overexpression. You can contact Genemedi for your unique reporter including GFP, zsgreen, RFP, mcherry or luciferase.The CMV promoter is a strong protomer driven broad expression in most tissues.You can easily produce a … login agfood.ieWebC3003-100μl. Lenti-EGFP-LC3B (10^9TU/ml) 100μl. 2992.00元. Lenti-EGFP-LC3B，即Lentivirus expressing EGFP-LC3B fusion protein，是一种可以表达EGFP-LC3B融合蛋白 … industry life cycle company examples