P53 antibody cst
WebThe permeabilized cells were then blocked with 0.1% bovine serum albumin and subsequently incubated with primary antibody of p-p53 (1:200) overnight at 4°C, followed by Alexa-488-labeled anti-rabbit IgG antibody (1:250) for 1 hour at room temperature. WebMar 4, 2024 · Antibody: Mouse monoclonal p53 Catalogue No: 2524 Clone: 1C12 Summary: Mouse monoclonal antibody raised against a synthetic peptide corresponding to residues surrounding Ser20 of human p53. Supplier recommended for WB, IP, IF, flow cytometry and ChIP. Reacts with human, mouse, rat, hamster and monkey p53. [enlarge] Figure 1.
P53 antibody cst
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WebApr 8, 2024 · Further immunofluorescent staining with p-p53 antibody showed that the p-p53 protein expression, which was mainly located in the cytoplasm of glomerular and tubular cells, was much higher in the kidney of the hyperuricemic mice than in the kidney of the control mice as well as the RHF and allopurinol treated mice (Figure 3H). These … WebOct 20, 2016 · The p53 Antibody was generated using tumor protein p53 as the antigen and it reacts with Human, and Non-Human Primate. p53 Antibody recognizes endogenous …
WebPhospho-p53 (Ser15) Antibody (MA5-15229) in ICC/IF. Immunofluorescence analysis of Phospho-p53 (Ser15) was performed using 70% confluent log phase HCT116 cells treated with etoposide. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 … WebAnti-p53 antibody [E26] (ab32389) Research with confidence – consistent and reproducible results with every batch Long-term and scalable supply – powered by recombinant technology for fast production Success from the first experiment – confirmed specificity through extensive validation Ethical standards compliant – production is animal-free
WebThe p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and … Webp53 (DO-1) is a mouse monoclonal antibody epitope mapping between amino acid residues 11-25 at the N-terminus of p53 of human origin. PRODUCT Each vial contains 200 µg IgG 2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Also available as TransCruz reagent for Gel ®
WebApr 16, 2024 · The tumor suppressor p53 is mutated in approximately half of all human cancers. p53 can induce apoptosis through mitochondrial membrane permeabilization by interacting with and antagonizing the...
WebImmunoprecipitation analysis of 200ug extracts of U-251MG cells using 3ug p53 antibody (A5761). Western blot was performed from the immunoprecipitate using p53 antibody (A5761) at a dilition of 1:1000. * For research use only. … palazzo social clubWebP53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2. 2. うどん工房 桜WebJun 19, 2006 · Monoclonal antibodies raised against p53 (pAb240 and pAb421), which recognize DNA-binding and C-terminal regions of p53 ( Bargonetti et al., 1993 ), were used … palazzo sofia licusatiWebThe p53 Antibody Sampler Kit provides an economical means of detecting p53 activity using modification-specific and control antibodies. The kit includes enough antibody to … うどん工房さぬき 林WebApr 1, 2024 · The early anti-p53 antibodies contributed to the recognition of p53 accumulation as a common feature of cancer cells and to our understanding of p53 DNA-binding and transcription activities. They led to the concept that conformational changes can facilitate p53's activity as a growth inhibitory protein. palazzo sofa bedWebProduct Name p53 (Ab-15) Antibody Host Species Rabbit Clonality Polyclonal Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. Applications WB IHC IF Species Reactivity Hu Ms Rt palazzo soderini frankensteinWebFeb 18, 2010 · The critical tumor suppressor p53 is mutated in over half of all human cancers. ... which was probed with a monoclonal antibody against p53 (pAb1801; Santa Cruz Biotechnology, Santa Cruz, CA), or β-actin (Santa Cruz Biotechnology), or γ-H2AX (Cell Signaling Technology). The membrane was subsequently probed with a horseradish … palazzo sofia pescara