Phospho-mtor antibody

Author: mktt

August undefined, 2024

WebA synthetic phospho-peptide corresponding to residues surrounding Ser2448 of human phospho mTOR. Formulation. 1X PBS, 0.09% NaN3, 0.2% BSA. Isotype. Rabbit IgGk. Preparation. Protein A+G. Recommended Usage. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. WebAnti-phospho-mTOR (Thr2446) Antibody Upstate®, from rabbit Synonym (s): FRAP1, FRAP, FRAP2, RAFT1, RAPT1, FK506 binding protein 12-rapamycin associated protein 1 eCl@ss: 32160702 NACRES: NA.41 Pricing and availability is not currently available. Properties biological source rabbit Quality Level 100 antibody form affinity purified immunoglobulin

Purified anti-mTOR Phospho (Ser2448) Antibody

WebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal ... WebDec 28, 2024 · Phospho-mTOR (Ser2481) Antibody Catalog Number: 2974S Clone Number: N/A Info: Supplier Page View Product Specs Browse Similar Products: MTOR Antibodies Cells (fibroblast, MRC-5 human cell lines) were starved form 4 hours (0,5% FBS) and induced with TGF-b. eIF4E siRNA was transfected with lipofectamine RNAmax for 24 hours. react react-quill

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WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. Web80596-1-RR targets Phospho-mTOR (Ser2448) in WB, ELISA applications and shows reactivity with Human, Rat samples. Background Information MTOR, also named as … WebAnti-mTOR (phospho T2446) antibody See all mTOR primary antibodies Description Rabbit polyclonal to mTOR (phospho T2446) Host species Rabbit Tested applications Suitable for: WB more details Species reactivity Reacts with: Mouse Immunogen Synthetic peptide corresponding to Human mTOR (phospho T2446). Database link: P42345 General notes react react-copy-to-clipboard

Phospho-mTOR (Ser2481) Antibody Affinity Biosciences

torc-specific phosphorylation of mtor phospho-ser2481 does

WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. Alias: Fold/Unfold WebKey features and details Rabbit polyclonal to mTOR (phospho S2448) Suitable for: WB, IHC-P, ICC/IF Reacts with: Human Isotype: IgG You may also be interested in Primary Anti … how to stay on call longer than 5 min aloneWebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. Alias: Fold/Unfold react react router dom

"WebInvitrogen Anti-Phospho-mTOR (Ser2448) Polyclonal, Catalog # PA5-85736. Tested in Western Blot (WB) and Immunohistochemistry (Paraffin) (IHC (P)) applications. This … " - Phospho-mtor antibody

Phospho-mtor antibody

Phospho-mTOR (Ser2448) antibody (67778-1-Ig)

WebRabbit Phospho-mTOR-S2448 Rabbit mAb (AP1413), validaed in WB,IHC and tested in Human,, Mouse. 100% Guaranteed. ABclonal provides trial size antibody samples for … WebProduct name Anti-mTOR (phospho S2481) antibody [EPR427 (N)] See all mTOR primary antibodies Description Rabbit monoclonal [EPR427 (N)] to mTOR (phospho S2481) Host species Rabbit Tested applications Suitable for: WB, Dot blot more details Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP Species reactivity Reacts with: Human

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WebFor Analyzing Cell Signaling Pathway Activation. Phosphorylation, the addition of a phosphate group to serine, threonine or tyrosine residues, is an important cellular process utilized to send cellular signals from the membrane to the nucleus. For example, changes in physiological conditions may require immediate changes in gene expression. WebAnti-mTOR Antibody detects level of phospho-mTOR (Thr2446) & has been published & validated for use in WB. Research Category Signaling Research Sub Category …

WebPhosphoPlus® mTOR (Ser2448) Antibody Duet PhosphoPlus ® mTOR (Ser2448) Antibody Duet #39182 Reviews () Citations (2) Flow cytometric analysis of A549 cells using mTOR (7C10) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (dashed line). WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for …

Webtracts were immunoprecipitated with rabbit -mTOR antibody or-AU1 antibody. Immune complexes were collected on protein A-Sepha-rose (Sigma) or -mouse IgG-agarose (Sigma), washed three times with ... tion—Previous reports demonstrated that mTOR was phospho-rylated in vitro by AKT at a serine residue (Ser-2448) located in studies, we ... WebAug 24, 2005 · Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody (ab1093) Formalin-fixed, paraffin-embedded human kidney tissue, (proximal …

WebULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to …

Webadult animals (c) Immunoprecipitation (IP) with mTOR antibody efficiently extracts mTOR complexes from prepared lysates. mTOR is highly enriched in the IP fraction but is absent in the supernatant and the wash fractions. Raptor is ... Phospho-mTOR (Ser-2884) and total mTOR antibodies were diluted 1:1000. Phospho-S6K (Thr-389), react react native 차이WebDescription: This MRRBY monoclonal antibody recognizes human and mouse mammalian target of rapamycin (also known as mTOR, FRAP, RAFT) when phosphorylated on S2448. … react re render on state changeWebDec 28, 2024 · Phospho-mTOR (Ser2481) Antibody Catalog Number: 2974S Clone Number: N/A Info: Supplier Page View Product Specs Browse Similar Products: MTOR Antibodies … react react must be in scope when using jsxWebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor … how to stay on a tubeWebPolyclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1872 publications. Validated for Western Blotting, Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-mTOR (Ser2448) Antibody (CST #2971) is ready to … react read file as base64Web4% PFA plus methanol. Methanol only. This clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and … react react-file-viewerWebAnti-phospho-mTOR (Ser2159) Antibody Anti-mTOR Antibody detects level of phospho-mTOR (Ser2159) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay, Dot Blot. - Find MSDS or SDS, a COA, data sheets and more information. react read csv